Andor Technology (AND) - United Kingdom

Supervisors: Peter Majer, Claire Greenwood

Student: open position

11 - Speckle analysis of actin and keratin filament dynamics

The pertinent difficulties within automated segmentation of filamentous networks can be avoided by fluorescent labeling of a small random filament fractions for speckle microscopy. We will apply speckle microscopy to study the dynamics of actin and keratin networks. We will work both on the imaging side and on the image processing side and on the interplay of the two to achieve reliable measurements, for example in the form of vector fields describing the dynamics of these networks. We expect that the standard tracking algorithms of the Imaris software can be improved for these applications. We will therefore investigate new and alternative tracking algorithms to deal with the large numbers of particles to be tracked.