The European network

for cell migration studies

Student: Laura Bornes

Supervisor: Jacco van RheenenRudolf LeubeReinhard Windoffer

7  -  Monitoring of cancer cell migration in living animals

In this project we will test whether epithelial mammary tumour cells need to remodel their actin cytoskeleton, or whether cells can also passively leave the primary tumour and establish metastases. Using intravital imaging techniques recently developed in the laboratory, the movement of individual tumour cells will be imaged in mammary tumours in living mice for several days through mammary optical imaging windows. Several components known to be required for actin remodelling will be genetically inhibited in the mammary tumour cells, and it will be tested how this affects active cell motility by intravital imaging. Image analysis tools developed by InCeM will be used for measuring cell motility. By inhibiting active cell migration, passive cell migration will become visible such as tumour cells that are pushed or pulled by immune cells, carried by interstitial fluid, or move due to tissue deformation. The importance of passive versus active cell migration for metastasis will be compared. This project will illustrate whether and to what extent passive cell migration is required for metastasis of mammary tumours, and which components are important for active cell migration.

Last update: 28.05.2018

Advanced cell migration assays (P1)

Chemotaxis and 2D/3D Migration (P2)

Analysis of keratin dynamics during migration (P3)

Impact of keratin network regulation on migrating cells (P4)

Correlation analyses of migration structure components and front-rear interplay (P5)

Life cycle analysis of actin, focal adhesions and force measurements (P6)

Monitoring of cancer cell migration in living animals  (P7)

Principles of the filopodia structure, dynamics and mechanics (P8)

Mechanisms of downstream signalling from the Rho GTPase network to

cell morphogenesis and cell motility (P9)

Real-time tracking of keratinocyte migration and analysis of cell membrane shape changes (P10)

Image analysis of integrated cytoskeletal network dynamics (P11)

Coupling bulk-surface models for cell migration (P12)

Shaping membranes and actin fibres by forces (P13)

Integrating shape change models and imaging – inverse problem solving and model validation (P14)

Understanding spatio-temporal dynamics of the cytosol network during cell migration  (P15)

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 642866.