The European network

for cell migration studies

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 642866.

3  -  Analysis of keratin dynamics during migration

We are interested in the role of keratin intermediate filaments during cell motility and the interdependence between keratin and the other cytoskeletal compounds, such as actin and microtubules. Keratin cycling in migrating cells and sessile cell will be quantified using image analysis tools. These values can be used for mathematical modeling of the transition from non-migratory to migratory. Chemical and genetic manipulations will be used in combination with novel imaging techniques to reconstruct the interactions between the cytoskeletal components. Together, this will lead to a better understanding in the cooperation between these components, needed for the highly regulated process of cell migration.

Maximum projection of actin (LifeAct-mRFP) and keratin intermediate filaments (K5-YFP) in a migrating nHEK cell.

Time-lapse recordings of HK18-YFP fluorescence (left) of a migrating EK18-1 cell displaying multiple emerging KFPs in the proceeding lamellipodium. (Kölsch et al., 2010)

Coupling bulk-surface models for cell migration (P12)

Shaping membranes and actin fibres by forces (P13)

Integrating shape change models and imaging – inverse problem solving and model validation (P14)

Understanding spatio-temporal dynamics of the cytosol network during cell migration  (P15)

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Last update: 19.12.2017