The European network

for cell migration studies

10 - Real-time tracking of keratinocyte migration and analysis
of cell membrane shape changes

Student: Katerina Lomanov

Supervisors: Hugh Gribben, Peter Majer, Jesus MartinezPaul Miller, Rudolf MerkelBernd Hoffmann

Imaging of moving cells requires continuous adjustments to keep cells and/or intracellular bodies of interest within the imaging field and in focus. We will develop methods to control microscopes via Andor's acquisition software to track the objects of interest in order to get better knowledge about cell migration, but also to keep them in the centre of the imaging field by performing continuous adjustments. Using available imaging data (JUELICH, UKA), we will investigate the use of tracking algorithms to compute movement and extract trajectories from multiple cells moving in a 3D environment. In order to automatically analyse local shape changes of single keratinocytes during migration, we will test algorithms to compute local growth rates and to detect newly emerging structures such as filopodia, lamellipodial protrusions, and fluorescently-labelled focal adhesions and cytoskeletal filaments from 2D and possibly 3D images of migrating cells. We will optimise ways to visualise the resulting information to make it easily accessible to the researcher. Shape descriptors will be defined to allow the characterisation and comparison of the evolution of cell shape during motion cycles of real and stimulated cells.

The main novelties we wish to introduce are the following:

• Provide a novel tracking framework with better performance, by investigating, among other elements, data association methods

• Develop a novel segmentation algorithm

• Integrate the tracking and segmentation in an efficient way

Last update: 28.05.2018

Advanced cell migration assays (P1)

Chemotaxis and 2D/3D Migration (P2)

Analysis of keratin dynamics during migration (P3)

Impact of keratin network regulation on migrating cells (P4)

Correlation analyses of migration structure components and front-rear interplay (P5)

Life cycle analysis of actin, focal adhesions and force measurements (P6)

Monitoring of cancer cell migration in living animals  (P7)

Principles of the filopodia structure, dynamics and mechanics (P8)

Mechanisms of downstream signalling from the Rho GTPase network to

cell morphogenesis and cell motility (P9)

Real-time tracking of keratinocyte migration and analysis of cell membrane shape changes (P10)

Image analysis of integrated cytoskeletal network dynamics (P11)

Coupling bulk-surface models for cell migration (P12)

Shaping membranes and actin fibres by forces (P13)

Integrating shape change models and imaging – inverse problem solving and model validation (P14)

Understanding spatio-temporal dynamics of the cytosol network during cell migration  (P15)

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 642866.