2 - Chemotaxis and 2D/3D Migration
Gradientech (GRAD) - Sweden
Student: Nikos Fatsis-Kavalopoulos
Supervisors: Sara Thorslund, Johan Kreuger
The focus of this project is to develop new methods based on microfluidic technology that will enable precise manipulation and positioning of cells, which will offer new possibilities to study fundamental mechanisms of epithelial and cancer cell migration. Cell communication and cell interactions that affect and guide cell migration will be studied in vitro using in house-developed state-of-the-art microfluidic chemotaxis assays. To this end, cell populations are to be exposed to gradients of single or multiple guidance cues with different orientation. We will determine how the cells respond with chemotaxis and/or chemokinesis. Migration will be monitored by using the tools provided and further developed through our network partners.
Last update: 28.05.2018
incem@rwth-aachen.de
Advanced cell migration assays (P1)
Chemotaxis and 2D/3D Migration (P2)
Analysis of keratin dynamics during migration (P3)
Impact of keratin network regulation on migrating cells (P4)
Correlation analyses of migration structure components and front-rear interplay (P5)
Life cycle analysis of actin, focal adhesions and force measurements (P6)
Monitoring of cancer cell migration in living animals (P7)
Principles of the filopodia structure, dynamics and mechanics (P8)
Mechanisms of downstream signalling from the Rho GTPase network to
cell morphogenesis and cell motility (P9)
Real-time tracking of keratinocyte migration and analysis of cell membrane shape changes (P10)
Image analysis of integrated cytoskeletal network dynamics (P11)
Coupling bulk-surface models for cell migration (P12)
Shaping membranes and actin fibres by forces (P13)
Integrating shape change models and imaging – inverse problem solving and model validation (P14)
Understanding spatio-temporal dynamics of the cytosol network during cell migration (P15)
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 642866.